Part 1
Purpose-to observe the growth of bacteria as well as find out how much bacteria are on everyday objects
Hypothesis- That section A which is a computer mouse will have the most bacteria because it is touched by many people many times throughout the day.
Materials-agar plate, Q-tip, objects to swab, inoculating loop, slide, microscope, crystal violet, iodine, ethanol, H20, safrinin, Bunsen burner, test tube, paper circles, bleach, kitchen cleaner, Windex
Method-get an agar plate and q-tips. Divide agar plate into 4 sections. Swab 4 objects and then swab agar plate to transfer bacteria. Place agar plate into incubator to grow bacteria. After a few days, take out bacteria and observe how each section grew and the characteristics of each section. Use inoculating loop to scrape off some bacteria and put on slide. Apply crystal violet and wait for 1-2 mins. rinse with H2O. apply iodine for 1-2 mins. rinse with H2O. wash with Ethanol until purple stops washing away, then rinse with H2O. apply safrinin for 1-2 mins. rinse with H2O and then blot dry. put slide under microscope and observe the colour and forms of bacteria.
Results-Plate 1, Section A-13 colonies,light yellow and creamy white,translucent, shiny, circular,convex, and entire.
Section B- many colonies, too many to count, creamy white, yellow,translucent, shiny, entire, punctiform, convex
Section C- 15 colonies, yellowish-white, translucent, shiny, entire, circular, and convex
Section D- 30 colonies, yellow, white, grey, translucent, some shiny, some dull, some entire, some lobate, some filamentous, some circular, some irregular, some convex, some flat.
Plate 2-Section E-6 colonies, yellowish-white, translucent, shiny, entire, circular, convex
Section F-12 colonies, yellowish-white, translucent, shiny, entire, circular, convex
Section G-15 colonies, yellowish-white, translucent, shiny,one dull, entire, one undulate, circular, one irregular, convex, one flat
Section H-15 colonies, yellowish-white, one completely white colony, translucent, one opaque, shiny, entire, circular, convex
Purpose-to observe the growth of bacteria as well as find out how much bacteria are on everyday objects
Hypothesis- That section A which is a computer mouse will have the most bacteria because it is touched by many people many times throughout the day.
Materials-agar plate, Q-tip, objects to swab, inoculating loop, slide, microscope, crystal violet, iodine, ethanol, H20, safrinin, Bunsen burner, test tube, paper circles, bleach, kitchen cleaner, Windex
Method-get an agar plate and q-tips. Divide agar plate into 4 sections. Swab 4 objects and then swab agar plate to transfer bacteria. Place agar plate into incubator to grow bacteria. After a few days, take out bacteria and observe how each section grew and the characteristics of each section. Use inoculating loop to scrape off some bacteria and put on slide. Apply crystal violet and wait for 1-2 mins. rinse with H2O. apply iodine for 1-2 mins. rinse with H2O. wash with Ethanol until purple stops washing away, then rinse with H2O. apply safrinin for 1-2 mins. rinse with H2O and then blot dry. put slide under microscope and observe the colour and forms of bacteria.
Results-Plate 1, Section A-13 colonies,light yellow and creamy white,translucent, shiny, circular,convex, and entire.
Section B- many colonies, too many to count, creamy white, yellow,translucent, shiny, entire, punctiform, convex
Section C- 15 colonies, yellowish-white, translucent, shiny, entire, circular, and convex
Section D- 30 colonies, yellow, white, grey, translucent, some shiny, some dull, some entire, some lobate, some filamentous, some circular, some irregular, some convex, some flat.
Plate 2-Section E-6 colonies, yellowish-white, translucent, shiny, entire, circular, convex
Section F-12 colonies, yellowish-white, translucent, shiny, entire, circular, convex
Section G-15 colonies, yellowish-white, translucent, shiny,one dull, entire, one undulate, circular, one irregular, convex, one flat
Section H-15 colonies, yellowish-white, one completely white colony, translucent, one opaque, shiny, entire, circular, convex
Analysis-Section D which was the phone in the Biology class was purple after gram staining, so it is gram positive which means it has a thin cell wall with lots of peptidoglycan. It also had three forms of bacteria, coccus, spirilli, and bacilli.
Conclusion-The results did not prove my hypothesis, as section D, which was the phone had the most bacteria growth, and not section A, which was the computer mouse. I might have gotten these results because the phone is also touched by many different people and objects, and probably not cleaned often so bacteria grows well on it. Some further experiments i could do are swabbing more mice in the computer lab and more phones in the classrooms, as then I could have a more accurate comparison as the mouse i swabbed could have been an abnormally clean mouse and the phone could have been an abnormally dirty phone.
Conclusion-The results did not prove my hypothesis, as section D, which was the phone had the most bacteria growth, and not section A, which was the computer mouse. I might have gotten these results because the phone is also touched by many different people and objects, and probably not cleaned often so bacteria grows well on it. Some further experiments i could do are swabbing more mice in the computer lab and more phones in the classrooms, as then I could have a more accurate comparison as the mouse i swabbed could have been an abnormally clean mouse and the phone could have been an abnormally dirty phone.
Part 2
Results-
Results-
Table for effectiveness of the cleaning products
Analysis-
1.There was a good lawn that covered pretty much all of the agar plate, with only 4 small empty sections, other than the zones of inhibition.
2. The agar plate gave nutrients to the bacteria to give them a place to grow. This experiment would not be possible without the agar plate as without it, the bacteria would have no nutrients. The experiment would also not work without the incubator as bacteria grow best in warmer conditions so without the incubator, the bacteria wouldn't have grown as well.
3.Kitchen cleaner worked the best because it has the largest zone of inhibition. This does not mean it will work against all bacteria because some bacteria may be immune to it, or more resistant to it.
4.Bleach because it had the smallest zone of inhibition, which was only slightly larger than the paper circle. This did surprise me because i thought bleach would work the best because it is a very strong substance that is very potent.
5. The experimental control was section D which had no paper disk in it. The purpose of this was to have a section where the bacteria grows with no chemicals so you can compare the control section with the sections with chemicals in them. We could tell if something was wrong in the experiment if the bacteria did not grow properly in the control section so we would know if it was a bad experiment
6. I would like to try a second experiment with the disks because some had too much liquid which ran off. Also i would like to try it again because bleach was supposed to be the most potent, but it wasn't for ours. So i want to know if we didn't do the experiment properly, or if our bacteria was resistant to the bleach.
7.This can happen by mutations happening in the DNA that could cause them to be immune, and then those with the mutation survive the antibiotics and the rest die off. So then eventually only the immune bacteria are left and they reproduce so eventually all of that bacteria will be resistant to that certain antibiotics.
8. Objects that were touched a lot, such as phones, computer mice, ATMs, and doorknobs. These surfaces are touched very often by many people every day, and not often cleaned so bacteria survive well on these surfaces.
1.There was a good lawn that covered pretty much all of the agar plate, with only 4 small empty sections, other than the zones of inhibition.
2. The agar plate gave nutrients to the bacteria to give them a place to grow. This experiment would not be possible without the agar plate as without it, the bacteria would have no nutrients. The experiment would also not work without the incubator as bacteria grow best in warmer conditions so without the incubator, the bacteria wouldn't have grown as well.
3.Kitchen cleaner worked the best because it has the largest zone of inhibition. This does not mean it will work against all bacteria because some bacteria may be immune to it, or more resistant to it.
4.Bleach because it had the smallest zone of inhibition, which was only slightly larger than the paper circle. This did surprise me because i thought bleach would work the best because it is a very strong substance that is very potent.
5. The experimental control was section D which had no paper disk in it. The purpose of this was to have a section where the bacteria grows with no chemicals so you can compare the control section with the sections with chemicals in them. We could tell if something was wrong in the experiment if the bacteria did not grow properly in the control section so we would know if it was a bad experiment
6. I would like to try a second experiment with the disks because some had too much liquid which ran off. Also i would like to try it again because bleach was supposed to be the most potent, but it wasn't for ours. So i want to know if we didn't do the experiment properly, or if our bacteria was resistant to the bleach.
7.This can happen by mutations happening in the DNA that could cause them to be immune, and then those with the mutation survive the antibiotics and the rest die off. So then eventually only the immune bacteria are left and they reproduce so eventually all of that bacteria will be resistant to that certain antibiotics.
8. Objects that were touched a lot, such as phones, computer mice, ATMs, and doorknobs. These surfaces are touched very often by many people every day, and not often cleaned so bacteria survive well on these surfaces.